Title
Light field microscopy
Abstract
By inserting a microlens array into the optical train of a conventional microscope, one can capture light fields of biological specimens in a single photograph. Although diffraction places a limit on the product of spatial and angular resolution in these light fields, we can nevertheless produce useful perspective views and focal stacks from them. Since microscopes are inherently orthographic devices, perspective views represent a new way to look at microscopic specimens. The ability to create focal stacks from a single photograph allows moving or light-sensitive specimens to be recorded. Applying 3D deconvolution to these focal stacks, we can produce a set of cross sections, which can be visualized using volume rendering. In this paper, we demonstrate a prototype light field microscope (LFM), analyze its optical performance, and show perspective views, focal stacks, and reconstructed volumes for a variety of biological specimens. We also show that synthetic focusing followed by 3D deconvolution is equivalent to applying limited-angle tomography directly to the 4D light field.
Year
DOI
Venue
2006
10.1145/1179352.1141976
ACM Trans. Graph.
Keywords
Field
DocType
tomography,show perspective view,optical performance,conventional microscope,light field,single photograph,focal stack,synthetic aperture,volume rendering.,perspective view,deconvolution,useful perspective view,light fields,microscopy,prototype light field microscope,biological specimen,volume rendering,light field microscopy,cross section
Microlens,Biological specimen,Computer graphics (images),Computer science,Optics,Deconvolution,Angular resolution,Light field,Microscope,Microscopy,Diffraction
Journal
Volume
Issue
ISSN
25
3
0730-0301
ISBN
Citations 
PageRank 
1-59593-364-6
36
2.81
References 
Authors
15
5
Name
Order
Citations
PageRank
Marc Levoy1102731073.33
Ren Ng290260.47
Andrew Adams393653.55
Matthew Footer4362.81
Mark Horowitz563741543.34