Title
Unravel four hairpins!
Abstract
DNA machines consisting of consecutive hairpins, which we have previously described, have various potential applications in DNA computation. In the present study, a 288-base DNA machine containing four consecutive hairpins was successfully constructed by ligation and PCR. PAGE and fluorescence spectroscopy experiments verified that all four hairpins were successfully opened by four opener oligomers, and that hairpin opening was dependent on the proper openers added in the correct order. Quantitative analysis of the final results by fluorescence spectroscopy indicated that all four hairpins were open in about 1/4 to 1/3 of the DNA machines.
Year
DOI
Venue
2008
10.1007/s11047-007-9035-y
Natural Computing
Keywords
Field
DocType
DNA computing,DNA nanotechnology,Molecular computing,Molecular memory,Molecular machine
Molecular machine,DNA machine,Discrete mathematics,DNA nanotechnology,Ligation,Molecular memory,Fluorescence spectroscopy,DNA,Computational biology,Bioinformatics,Mathematics,DNA computing
Journal
Volume
Issue
Citations 
7
2
2
PageRank 
References 
Authors
0.39
6
5
Name
Order
Citations
PageRank
Atsushi Kameda1629.23
Masahito Yamamoto220.39
Azuma Ohuchi338668.99
Satsuki Yaegashi4366.10
Masami Hagiya5649102.85