Title
Automatic Acquisition of Immunofluorescence Images: Algorithms and Evaluation
Abstract
In this paper, we report our experience in the development of a system for automatic acquisition of Immuno-Fluorescence Assay (IFA) images. We focus on two basic issues. Firstly, we determine an autofocus function that can deal with photobleaching, a physical phenomenon affecting automatic acquisition of IFA images, and present a set of experiments on real images that confirm its effectiveness. Secondly, we discuss if the physicians may reliably use digital IFA images in place of direct microscope observations to carry out the diagnosis. In this respect, we present the results of a preliminary experiment where physicians perform the diagnosis on a set of images both by looking directly to them at the fluorescence microscope and by looking at digital images on the screen of a workstation.
Year
DOI
Venue
2006
10.1109/CBMS.2006.53
CBMS
Keywords
Field
DocType
fluorescence microscope,automatic acquisition,physical phenomenon,digital image,ifa image,direct microscope observation,immunofluorescence images,immuno-fluorescence assay,basic issue,autofocus function,digital ifa image,photobleaching
Immunofluorescence,Computer vision,Autofocus,Computer science,Workstation,Digital image,Microscope,Artificial intelligence,Real image
Conference
ISSN
ISBN
Citations 
2372-9198
0-7695-2517-1
2
PageRank 
References 
Authors
0.58
1
4
Name
Order
Citations
PageRank
Paolo Soda140739.44
Amelia Rigon230.95
Antonella Afeltra320.58
Giulio Iannello441446.75