Paper Info
Title
Structural Role of Uracil DNA Glycosylase for the Recognition of Uracil in DNA Duplexes. Clues from Atomistic Simulations.
Abstract
In the first stage of the base excision repair pathway the enzyme uracil DNA glycosylase (UNG) recognizes and excises uracil (U) from DNA filaments. U repair is believed to occur via a multistep base-flipping process, through which the damaged U base is initially detected and then engulfed into the enzyme active site, where it is cleaved. The subtle recognition mechanism by which UNG discriminates between U and the other similar pyrimidine nucleobases is still a matter of active debate. Detailed structural information on the different steps of the base-flipping pathway may provide insights on it. However, to date only two intermediates have been trapped crystallographically thanks to chemical modifications of the target and/or of its complementary base. Here, we performed force-field based molecular dynamics (MD) simulations to explore the structural and dynamical properties of distinct UNG/dsDNA adducts, containing A:U, A:T, G:U, or G:C base pairs, at different stages of the base-flipping pathway. Our simulations reveal that if U is present in the DNA sequence a short-lived extra-helical (EH) intermediate exists. This is stabilized by a water-mediated H-bond network, which connects U with His148, a residue pointed out by mutational studies to play a key role for U recognition and catalysis. Moreover, in this EH intermediate, UNG induces a remarkable overall axis bend to DNA. We believe this aspect may facilitate the flipping of U, with respect to other similar nucleobases, in the latter art of the base-extrusion process. In fact, a large DNA bend has been demonstrated to be associated with a lowering of the free energy barrier for base-flipping. A detailed comparison of our results with partially flipped intermediates identified crystallographically or computationally for other base-flipping enzymes allows us to validate our results and to formulate hypothesis on the recognition mechanism of LING. Our study Provides a first ground for a detailed understanding of the LING repair pathway, which is necessary to devise new pharmaceutical strategies for targeting DNA-related pathologies.
Year
DOI
Venue
2013
10.1021/ci4001647
JOURNAL OF CHEMICAL INFORMATION AND MODELING
Field
DocType
Volume
Enzyme,Nucleobase,Pyrimidine,Combinatorial chemistry,Uracil,Chemistry,DNA,Active site,Stereochemistry,Base pair,Uracil-DNA glycosylase
Journal
53
Issue
ISSN
Citations 
6
1549-9596
0
PageRank 
References 
Authors
0.34
4
4
Name
Order
Citations
PageRank
Duvan Franco100.34
Jacopo Sgrignani2123.38
Giovanni Bussi311210.81
Alessandra Magistrato473.69