Paper Info
Title
Tandem duplication, circular permutation, molecular adaptation: how Solanaceae resist pests via inhibitors.
Abstract
The Potato type II (Pot II) family of proteinase inhibitors plays critical roles in the defense system of plants from Solanaceae family against pests. To better understand the evolution of this family, we investigated the correlation between sequence and structural repeats within this family and the evolution and molecular adaptation of Pot II genes through computational analysis, using the putative ancestral domain sequence as the basic repeat unit.Our analysis discovered the following interesting findings in Pot II family. (1) We classified the structural domains in Pot II family into three types (original repeat domain, circularly permuted domain, the two-chain domain) according to the existence of two linkers between the two domain components, which clearly show the circular permutation relationship between the original repeat domain and circularly permuted domain. (2) The permuted domains appear more stable than original repeat domain, from available structural information. Therefore, we proposed a multiple-repeat sequence is likely to adopt the permuted domain from contiguous sequence segments, with the N- and C-termini forming a single non-contiguous structural domain, linking the bracelet of tandem repeats. (3) The analysis of nonsynonymous/synonymous substitution rates ratio in Pot II domain revealed heterogeneous selective pressures among amino acid sites: the reactive site is under positive Darwinian selection (providing different specificity to target varieties of proteinases) while the cysteine scaffold is under purifying selection (essential for maintaining the fold). (4) For multi-repeat Pot II genes from Nicotiana genus, the proteolytic processing site is under positive Darwinian selection (which may improve the cleavage efficiency).This paper provides comprehensive analysis and characterization of Pot II family, and enlightens our understanding on the strategies (Gene and domain duplication, structural circular permutation and molecular adaptation) of Solanaceae plants for defending pathogenic attacks through the evolution of Pot II genes.
Year
DOI
Venue
2008
10.1186/1471-2105-9-S1-S22
BMC Bioinformatics
Keywords
Field
DocType
tandem repeat,microarrays,mutation,tandem duplication,bioinformatics,algorithms,structure activity relationship,amino acid,purifying selection
Repeat unit,Gene,Biology,Solanaceae,Bioinformatics,DNA Mutational Analysis,Genetics,Tandem exon duplication,Circular permutation in proteins,DNA microarray,Mutation
Journal
Volume
Issue
ISSN
9 Suppl 1
S-1
1471-2105
Citations 
PageRank 
References 
28
0.41
12
Authors
2
Name
Order
Citations
PageRank
Lesheng Kong1352.01
Shoba Ranganathan268936.60