Paper Info
Title
An effective mutation-based measure for evaluating the suitability of parental sequences to undergo DNA shuffling experiments
Abstract
The DNA shuffling process has been successfully used in many experiments of Directed Molecular Evolution. In a shuffling experiment genes are recombined by an iterative procedure of PCR cycles aiming at obtaining new genes, hopefully with some of the original functions being improved. The optimizations of the parameters involved in the process as well as the characteristics of the parental sequences are of extreme importance to guarantee the success of a shuffling experiment. This paper proposes a new measure, based on the number of bases between existing mutations in the parental sequences, suitable for evaluating the suitability of two sequences to be submitted to a DNA shuffling experiment. In order to investigate the usefulness of the proposed mutation-based measure versus two commonly used measures, a family of 37 DNA gene sequences codifying for snake venom metallopeptidases was used for evaluation purposes using the three measures. The parental sequences identified by each of the three measures were validated by simulating the DNA shuffling process using the software eShuffle. The eShuffle results illustrate on the benefits of the mutation-based measure proposed in this paper.
Year
DOI
Venue
2008
10.1109/CEC.2008.4630882
IEEE Congress on Evolutionary Computation
Keywords
DocType
Citations 
DNA,biology computing,genetic algorithms,genetics,iterative methods,molecular biophysics,proteins,DNA gene sequences,DNA shuffling experiments,DNA shuffling process,PCR cycles,directed molecular evolution,eShuffle,iterative procedure,mutation-based measure,parental sequences,snake venom metallopeptidases
Conference
1
PageRank 
References 
Authors
0.63
4
4
Name
Order
Citations
PageRank
Luciana Montera111.64
Maria Do Carmo Nicoletti251.80
Flavio Henrique Da Silva310.96
Pablo Moscato433437.27