Paper Info
Title
Optimizing ethanol production selectivity
Abstract
Lactococcus lactis metabolizes glucose homofermentatively to lactate. However, after disruption of the gene coding for lactate dehydrogenase, LDH, a key enzyme in NAD^+ regeneration, the glycolytic flux shifts from homolactic to mixed-acid fermentation with the redirection of pyruvate towards production of formate, acetate, ethanol and CO"2. A mathematical model of the pyruvate metabolism pathway that enhances ethanol production was developed from in vivo Nuclear Magnetic Resonance (NMR) time-series measurements that describe the dynamics of the metabolites in L. lactis. Both Michaelis-Menten and S-system models capture the observed in vivo dynamics of the glycolysis pathway in L. lactis, while prior models describe only the in vitro dynamics. The models provide insight into the maximization of selectivity of ethanol with respect to acetate and CO"2 as undesired products in multiple reactions. High concentrations of NADH and acetyl-CoA and low concentrations of pyruvate and NAD appear to maximize ethanol selectivity.
Year
DOI
Venue
2011
10.1016/j.mcm.2010.01.016
Mathematical and Computer Modelling
Keywords
Field
DocType
ethanol selectivity,michaelis–menten,s-system model,lactate dehydrogenase,selectivity maximization,ethanol production,optimizing ethanol production selectivity,l. lactis,glycolysis pathway,vivo dynamic,vivo nuclear magnetic resonance,lactococcus lactis metabolizes glucose,s -systems,pyruvate metabolism pathway,system modeling,mathematical model,metabolic pathway,s,nuclear magnetic resonance,time series,michaelis menten,enzyme
Ethanol fuel,Mathematical analysis,Biochemistry,Ethanol,Lactococcus lactis,Fermentation,Glycolysis,Lactate dehydrogenase,Mathematics,NAD+ kinase,Formate
Journal
Volume
Issue
ISSN
53
7-8
Mathematical and Computer Modelling
Citations 
PageRank 
References 
0
0.34
4
Authors
4
Name
Order
Citations
PageRank
Raman Lall1101.64
Timothy J Donohue2171.95
Simeone Marino3314.35
Julie C. Mitchell414663.99