Paper Info
Title
Towards fully automated phototransfection
Abstract
Flexible automation technologies have been applied to the manual phototransfection procedure on fibroblast and astrocyte cells.We have designed and implemented a framework for increased throughput of the entire process. Integrated image processing, laser target position calculation, and stage movements show a throughput increase of 23X over the current manual method while the potential for even greater throughput improvements ( 110X) is described. A software tool for automated single cell morphological measurements has also been constructed and shown to be able quantify changes in the cell before and after the process, successfully characterizing them, using metrics such as cell perimeter, area, major and minor axis length, and eccentricity values.
Year
DOI
Venue
2009
10.1109/COASE.2009.5234165
CASE
Keywords
Field
DocType
eccentricity value,automated single cell morphological,throughput increase,cell perimeter,manual phototransfection procedure,greater throughput improvement,entire process,automated phototransfection,increased throughput,astrocyte cell,current manual method,computer languages,process integration,stem cells,automation,graphical user interfaces,image processing,pixel,image segmentation
Eccentricity (behavior),Computer science,Simulation,Image processing,Image segmentation,Automation,Graphical user interface,Software,Pixel,Throughput,Computer hardware
Conference
ISSN
ISBN
Citations 
2161-8070
978-1-4244-4579-0
0
PageRank 
References 
Authors
0.34
10
6
Name
Order
Citations
PageRank
David J. Cappelleri19914.02
Ádám Halász220313.41
Jai-Yoon Sul300.34
Tae-Kyung Kim45610.06
James Eberwine500.34
Vijay Kumar61158187.91