Title
Probabilistic Tracking of Virus Particles in Fluorescence Microscopy Image Sequences
Abstract
Fluorescence time-lapse microscopy is a powerful technique for observing the spatial-temporal behavior of viruses. To quantitatively analyze the exhibited dynamical relationships, tracking of viruses over time is required. We developed probabilistic approaches based on parti- cle filters for tracking multiple virus particles in time-lapse fluorescence microscopy images. We employed a mixture of particle filters as well as independent particle filters. For the latter, we have developed a penal- ization strategy to maintain the identity of the tracked objects in cases where objects are in close proximity. We have applied the approaches to synthetic images and quantified their performance. We have also success- fully applied the approaches to real microscopy images of HIV-1 particles and have compared the tracking results with ground truth from manual tracking.
Year
DOI
Venue
2008
10.1007/978-3-540-78640-5_90
Bildverarbeitung für die Medizin
Keywords
Field
DocType
ground truth,particle filter,fluorescence microscopy
Computer vision,Fluorescence microscope,Particle filter,Fluorescence,Ground truth,Artificial intelligence,Probabilistic logic,Microscopy,Physics
Conference
Citations 
PageRank 
References 
2
0.44
4
Authors
6
Name
Order
Citations
PageRank
William J. Godinez19110.98
Marko Lampe2464.16
Stefan Wörz325632.58
Barbara Müller4173.02
Roland Eils564470.09
Karl Rohr634048.69