Name
Title | ||
|---|---|---|
Selective Adsorption Of An Antibody Onto A Plasma-Polymerized Film For Protein Patterning |
Abstract | ||
|---|---|---|
Techniques for patterned modification of substrate surfaces are important for forming microarrays on protein chips. A hexamethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and the resulting surface was partially modified by subsequent nitrogen plasma treatment with a patterned shadow mask. When surface adsorption of an antibody protein (F(ab')(2) fragment of anti-human immunoglobulin G) was visualized by fluorescence microscopy, distinct 80 X 80 mu m(2) square spots were observed, surrounded by a non-fluorescent 80 mu m-wide grid. This pattern could be attributed to proteins selectively adsorbed onto the nitrogen plasma-treated regions but not onto the surface of pristine HMDS PPF. This provided a simple fabrication method of protein patterning. |
Year | DOI | Venue |
|---|---|---|
2008 | 10.1093/ietele/e91-c.6.978 | IEICE TRANSACTIONS ON ELECTRONICS |
Keywords | Field | DocType |
antibody, F(ab')(2) fragment, plasma-polymerized film, adsorption, patterning | Substrate (chemistry),Fluorescence microscope,Nanotechnology,Hexamethyldisiloxane,Shadow mask,Fluorescence,Polymerization,Selective adsorption,Optics,Chemical engineering,Engineering,Adsorption | Journal |
Volume | Issue | ISSN |
E91C | 6 | 0916-8524 |
Citations | PageRank | References |
1 | 0.63 | 0 |
Authors | ||
4 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Atsunori Hiratsuka | 1 | 1 | 0.63 |
| Naoya Murata | 2 | 1 | 1.64 |
| hitoshi | 3 | 1 | 4.01 |
| Kazunari Matsumura | 4 | 1 | 0.63 |