Name
Title | ||
|---|---|---|
Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery |
Abstract | ||
|---|---|---|
The purpose of this study was to test the feasibility of using a novel biodegradable microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. We first encapsulated green fluorescent protein (GFP) gene into the microspheres and validated the echogenic property of GFP-plasmid/microspheres with intramuscular injection. Then, the GFP-plasmid/microspheres were transferred into the arteries of pigs via a catheter-based delivery procedure under ultrasound imaging. A strong echogenic signal reflected from the microspheres indicated the destination of genes delivered, whereas functional gene expression was confirmed by immunohistochemical examination. Microspheres can be feasible contrast agents/gene vectors in ultrasound imaging-based vascular gene delivery in vivo. |
Year | DOI | Venue |
|---|---|---|
2002 | 10.1109/ISBI.2002.1029429 | ISBI |
Keywords | Field | DocType |
functional gene expression,gene destination,contrast agent,arteries,genetics,blood vessels,ultrasound imaging-based vascular gene delivery,cardiovascular diseases,proteins,biomedical ultrasonics,pigs,gene vector,in vivo,fluorescence,biochemistry,cardiovascular system,immunohistochemical examination,gfp-plasmid/microspheres,green fluorescent protein gene,vascular gene therapy,strong echogenic signal,catheter-based delivery procedure,echogenic property,patient treatment,biodegradable microsphere,intramuscular injection,catheters,green fluorescent protein,gene expression,polymers,gene delivery,immune system | Gene,Gene delivery,Computer science,Gene expression,Echogenicity,In vivo,Plasmid,Delivery Procedure,Pathology,Green fluorescent protein | Conference |
ISBN | Citations | PageRank |
0-7803-7584-X | 0 | 0.34 |
References | Authors | |
0 | 10 | |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Xiangying Du | 1 | 0 | 1.69 |
| Yuesong Yang | 2 | 1 | 1.11 |
| Catherine Le Visage | 3 | 0 | 0.68 |
| Hunter H. Chen | 4 | 0 | 1.01 |
| Robert Dejong | 5 | 0 | 0.34 |
| Bensheng Qiu | 6 | 11 | 6.59 |
| Danming Wang | 7 | 0 | 0.34 |
| Kam W. Leong | 8 | 1 | 2.33 |
| Ulrike M. Hamper | 9 | 1 | 1.42 |
| Xiaoming Yang | 10 | 0 | 2.03 |