Paper Info
Title
Battery-powered miniaturized electroporation system for in-situ gene transfer in stem cell research
Abstract
A battery-powered miniaturized electroporation (EP) system was designed and fabricated to tackle the challenging DNA transfection of stem cells. The system consists of a programmable mini-electroporator and a micro EP chip. Large statistical data of loading efficiency for five different size molecules and cell viability were determined under various voltage and duration in this system for HeLa cell and MEF cell line. Based on more than 2,000 single-cell electroporation experiments, an EP phase diagram for MEF cells have been generated for the first time. It can be used to significantly increase the in-situ gene transfer efficiency. In addition, the EP boundary curve for MEF cells can be described by a simple empirical formula. Comparing to the conventional instrument, the miniaturized electroporation system can greatly shorten the experimental time. We also proposed a new system for continuous online monitoring in-situ gene expression, which is still in progress,
Year
DOI
Venue
2009
10.1109/NEMS.2009.5068619
NEMS
Keywords
DocType
ISSN
ep phase diagram,cell viability,hela cell,in-situ gene transfer,mef cell,stem cell research,ep boundary curve,electroporation system,single-cell electroporation experiment,new system,micro ep chip,mef cell line,fabrication,electroporation,gene transfer,gene expression,dna,cell line,biomems,stem cells,fluorescence,embryo,proteins,stem cell,gold,phase diagram,chip,data mining,probability density function,electric fields
Conference
2474-3747
Citations 
PageRank 
References 
1
0.71
0
Authors
3
Name
Order
Citations
PageRank
Huiqi He110.71
Donald C. Chang211.72
Yi-Kuen Lee3711.16