Title
Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.
Abstract
The DNA sequence of a 225.4 kilobase segment of the Escherichia coli K-12 genome is described here, from 76.0 to 81.5 minutes on the genetic map. This brings the total of contiguous sequence from the E.coli genome project to 725.1 kb(76.0 to 92.8 minutes). We found 191 putative coding genes (ORFs) of which 72 genes were previously known, and 110 of which remain unidentified despite literature and similarity searches. Seven new genes-arsE, arsF, arsG, treF, xylR, xylG, and xylH-were identified as well as the previously mapped pit and dctA genes. The arrangement of proposed genes relative to possible promoters and terminators suggests 90 potential transcription units. Other features include 19 REP elements, 95 computer-predicted bends, 50 Chi sites, and one grey hole. Thirty-one putative signal peptides were found, including those of thirteen known membrane or periplasmic proteins. One tRNA gene (proK) and two insertion sequences (IS5 and IS150) are located in this segment. The genes in this region are organized with equal numbers oriented with or against replication.
Year
DOI
Venue
1994
10.1093/nar/22.13.2576
NUCLEIC ACIDS RESEARCH
DocType
Volume
Issue
Journal
22
13.0
ISSN
Citations 
PageRank 
0305-1048
7
2.17
References 
Authors
0
5
Name
Order
Citations
PageRank
Heidi J. Sofia1677.22
Valerie Burland2122.85
Donna L. Daniels372.17
Guy Plunkett4727.65
Frederick R. Blattner5278193.27