Name
Abstract | ||
|---|---|---|
The Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is a relatively simple and inexpensive method for genotyping single nucleotide polymorphisms (SNPs). It requires minimal investment in instrumentation. Here, we describe a web application, 'SNP Cutter,' which designs PCR-RFLP assays on a batch of SNPs from the human genome. NCBI dbSNP rs IDs or formatted SNPs are submitted into the SNP Cutter which then uses restriction enzymes from a pre-selected list to perform enzyme selection. The program is capable of designing primers for either natural PCR-RFLP or mismatch PCR-RFLP, depending on the SNP sequence data. SNP Cutter generates the information needed to evaluate and perform genotyping experiments, including a PCR primers list, sizes of original amplicons and different allelic fragment after enzyme digestion. Some output data is tab-delimited, therefore suitable for database archiving. The SNP Cut-ter is available at http://bioinfo.bsd.uchicago.edu/SNP_cutter.htm. |
Year | DOI | Venue |
|---|---|---|
2005 | 10.1093/nar/gki358 | NUCLEIC ACIDS RESEARCH |
Keywords | Field | DocType |
polymerase chain reaction,restriction fragment length polymorphism,human genome,enzyme,restriction enzyme,single nucleotide polymorphism | Genotyping,Biology,Restriction fragment length polymorphism,dbSNP,SNP genotyping,Single-nucleotide polymorphism,Human genome,Genetics,SNP,Molecular biology,Molecular Inversion Probe | Journal |
Volume | Issue | ISSN |
33 | SUPnan | 0305-1048 |
Citations | PageRank | References |
7 | 0.56 | 2 |
Authors | ||
8 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Ruifang Zhang | 1 | 9 | 2.07 |
| Zanhua Zhu | 2 | 9 | 1.06 |
| Hongming Zhu | 3 | 9 | 3.29 |
| Tu Nguyen | 4 | 10 | 2.26 |
| Fengxia Yao | 5 | 9 | 1.06 |
| Kun Xia | 6 | 21 | 3.91 |
| Desheng Liang | 7 | 7 | 0.56 |
| Chunyu Liu | 8 | 12 | 4.45 |