Paper Info
Title
Biased Reptation Model With Electroosmosis For Dna Electrophoresis In Microchannels With A Sub-Micron Pillar Array
Abstract
A novel biased reptation model with electroosmosis (BRE) is proposed for a long-chain DNA electrophoresis separation mechanism in microchannels with a sub-micron pillar array. The BRE model incorporates effective electroosmosis into the classical biased reptation model (BRM). Initial investigation of the BRM with additional electroosmosis flow was conducted. In order to validate the proposed BRE model, the apparent electrophoretic mobilities of different sizes of long-chain DNA, T4 DNA and lambda DNA at different electric fields were measured in a microfabricated electrophoresis chip with an embedded sub-micron pillar array. The current-monitoring method was used to measure the electroosmotic mobility in the microfabricated electrophoresis chip. The proposed BRE model shows much better agreement with the experimental results compared to the classical BRM and the semi-empirical results based on the biased reptation with fluctuation (BRF). The average standard error between our proposed BRE model and experimental data is 8.13% without any fitting parameters, while the errors are 343.01% for the BRM and 17.54% for the BRF with one fitting parameter, respectively.
Year
DOI
Venue
2010
10.1088/0960-1317/21/8/085031
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
Keywords
Field
DocType
electrophoresis,osmosis,molecular biophysics,electrophoretic mobility,electric field,electroosmosis,force,dna electrophoresis,electric fields,eof,lab on a chip,dna
Analytical chemistry,Electric field,Micrometre,Gel electrophoresis of nucleic acids,Reptation,Electrophoresis,DNA,Lab-on-a-chip,Pillar,Materials science
Conference
Volume
Issue
ISSN
21
8
0960-1317
Citations 
PageRank 
References 
0
0.34
0
Authors
3
Name
Order
Citations
PageRank
Wentao Wang100.34
Yick Chuen Chan221.57
Yi-Kuen Lee3711.16