Name
Title | ||
|---|---|---|
Zinc Finger Database (ZiFDB): a repository for information on C2H2 zinc fingers and engineered zinc-finger arrays. |
Abstract | ||
|---|---|---|
Zinc fingers are the most abundant DNA-binding motifs encoded by eukaryotic genomes and one of the best understood DNA-recognition domains. Each zinc finger typically binds a 3-nt target sequence, and it is possible to engineer zinc-finger arrays (ZFAs) that recognize extended DNA sequences by linking together individual zinc fingers. Engineered zinc-finger proteins have proven to be valuable tools for gene regulation and genome modification because they target specific sites in a genome. Here we describe ZiFDB (Zinc Finger Database; http://bindr.gdcb.iastate.edu/ZiFDB), a web-accessible resource that compiles information on individual zinc fingers and engineered ZFAs. To enhance its utility, ZiFDB is linked to the output from ZiFiT-a software package that assists biologists in finding sites within target genes for engineering zinc-finger proteins. For many molecular biologists, ZiFDB will be particularly valuable for determining if a given ZFA (or portion thereof) has previously been constructed and whether or not it has the requisite DNA-binding activity for their experiments. ZiFDB will also be a valuable resource for those scientists interested in better understanding how zinc-finger proteins recognize target DNA. |
Year | DOI | Venue |
|---|---|---|
2009 | 10.1093/nar/gkn606 | NUCLEIC ACIDS RESEARCH |
Keywords | Field | DocType |
dna binding proteins,zinc fingers,transcription factors,protein engineering,zinc finger protein,dna sequence,zinc finger,web accessibility,binding sites,dna,gene regulation | Genome,Gene,Biology,Protein engineering,DNA-binding protein,DNA,DNA sequencing,Zinc finger,C2H2 Zinc Finger,Genetics,Molecular biology,Database | Journal |
Volume | Issue | ISSN |
37 | Database issue | 0305-1048 |
Citations | PageRank | References |
9 | 2.58 | 2 |
Authors | ||
8 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Fengli Fu | 1 | 18 | 3.26 |
| Jeffry Sander | 2 | 71 | 8.89 |
| Morgan Maeder | 3 | 28 | 4.39 |
| Stacey Thibodeau-Beganny | 4 | 18 | 2.93 |
| J Keith Joung | 5 | 31 | 6.08 |
| Drena Dobbs | 6 | 423 | 35.43 |
| Leslie L. Miller | 7 | 43 | 11.55 |
| Daniel F Voytas | 8 | 61 | 9.41 |