Name
Title | ||
|---|---|---|
Vision in the Small: Reconstructing the Structure of Protein Macromolecules from Cryo-Electron Micrographs |
Abstract | ||
|---|---|---|
Single particle reconstruction using Cryo-Electron Microscopy (cryo-EM) is an emerging technique in structural biology for estimating the 3-D struc- ture (density) of protein macromolecules. Unlike tomography where a large number of images of a specimen can be acquired, the number of images of an individual particle is limited because of radiation damage. Instead, the specimen consists of identical copies of the same protein macro-molecule embedded in vitreous ice at random and unknown 3-D orientations. Because the images are extremely noisy, thousands to hundreds-of-thousands of pro- jections are needed to achieve the desired resolution of 5 A. Along with dif- ferences of the imaging modality compared to photographs, single particle reconstruction provides a unique set of challenges to existing computer vi- sion algorithms. Here, we introduce the challenge and opportunity of recon- struction from transmission electron micrographs, and briey describe our contributions in areas of particle detection, contrast transfer function (CTF) estimation, and initial 3-D model construction. |
Year | Venue | Keywords |
|---|---|---|
2006 | BMVC | contrast transfer function,radiation damage,structural biology,cryo electron microscopy |
Field | DocType | Citations |
Computer vision,Computer science,Structural biology,Data acquisition,Tomography,Robustness (computer science),Artificial intelligence,Microscopy,Cryo-electron microscopy,Electron,Contrast transfer function | Conference | 0 |
PageRank | References | Authors |
0.34 | 1 | 4 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Satya P. Mallick | 1 | 228 | 10.70 |
| Sameer Agarwal | 2 | 10328 | 478.10 |
| David Kriegman | 3 | 7693 | 451.96 |
| Serge J. Belongie | 4 | 12512 | 1010.13 |