Name
Title | ||
|---|---|---|
Analysis of context of 5'-splice site sequences in mammalian mRNA precursors by subclass method. |
Abstract | ||
|---|---|---|
The signals that direct the excision of introns from mammalian pre-mRNA are not yet well understood. However, at least three kinds of signals--5'-splice site signals, 3'-splice site signals and branch point signals--play important roles in the excision of introns. In the present paper we treat only the 5'-splice sites. In addition to a consensus sequence for 5'-splice signals, several methods have been proposed, based on a statistical model, and used to analyze relative importance of each nucleotide at each position. In our approach a nucleotide sequence is regarded as a string with symbols of 'A', 'T', 'G' and 'C'; important substrings of 5'-splice site sequences, called pattern sequences, are extracted. A pattern sequence expresses which nucleotide is needed at a limited number of positions around the 5'-splice site. It is observed that a particular pattern sequence matches predominantly 5'-splice site sequences nearest to the 5'-end of a gene and another pattern sequence matches predominantly the second nearest ones. Moreover, it is confirmed that the pattern sequences accurately predict authentic 5'-splice sites for unknown genes and explain some mutation examples. |
Year | DOI | Venue |
|---|---|---|
1992 | 10.1093/bioinformatics/8.4.367 | Computer Applications in the Biosciences |
Field | DocType | Volume |
Subclass,Biology,splice,Nucleic acid sequence,Messenger RNA,Intron,RNA splicing,Bioinformatics,Splice site mutation,Consensus sequence | Journal | 8 |
Issue | ISSN | Citations |
4 | 0266-7061 | 2 |
PageRank | References | Authors |
0.64 | 0 | 4 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Mineichi Kudo | 1 | 927 | 116.09 |
| S Kitamura-Abe | 2 | 5 | 1.15 |
| Masaru Shimbo | 3 | 179 | 33.02 |
| Y Lida | 4 | 3 | 1.01 |