Name
Title | ||
|---|---|---|
EMatch: Discovery of High Resolution Structural Homologues of Protein Domains in Intermediate Resolution Cryo-EM Maps |
Abstract | ||
|---|---|---|
Cryo-EM has become an increasingly powerful technique for elucidating the structure, dynamics, and function of large flexible macromolecule assemblies that cannot be determined at atomic resolution. However, due to the relatively low resolution of cryo-EM data, a major challenge is to identify components of complexes appearing in cryo-EM maps. Here, we describe EMatch, a novel integrated approach for recognizing structural homologues of protein domains present in a 6-10 Å resolution cryo-EM map and constructing a quasi-atomic structural model of their assembly. The method is highly efficient and has been successfully validated on various simulated data. The strength of the method is demonstrated by a domain assembly of an experimental cryo-EM map of native GroEL at 6 Å resolution. |
Year | DOI | Venue |
|---|---|---|
2007 | 10.1109/TCBB.2007.1003 | IEEE/ACM Trans. Comput. Biology Bioinform. |
Keywords | Field | DocType |
high resolution,structural dynamics,cyclic symmetry,low resolution,atomic structure,secondary structure,molecular biophysics,proteins,protein domains,structural bioinformatics | Structural bioinformatics,Protein domain,Computer science,Cyclic symmetry,GroEL,Molecular biophysics,Bioinformatics,Cryo-electron microscopy | Journal |
Volume | Issue | ISSN |
4 | 1 | 1545-5963 |
Citations | PageRank | References |
16 | 2.84 | 5 |
Authors | ||
5 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Keren Lasker | 1 | 21 | 4.59 |
| Oranit Dror | 2 | 136 | 12.48 |
| Maxim Shatsky | 3 | 127 | 13.11 |
| Ruth Nussinov | 4 | 845 | 113.97 |
| Haim J Wolfson | 5 | 1837 | 495.25 |