Name
Title | ||
|---|---|---|
The development of a proteomic analyzing pipeline to identify proteins with multiple RRMs and predict their domain boundaries |
Abstract | ||
|---|---|---|
The RNA-recognition motif (RRM) is the most abundant RNA-binding domain involved in many post-transcriptional processes. Since RRM-containing proteins have different functions with similar domain architecture, it is challenging to implement an automated annotation tool for these proteins in proteomic analysis. In this study, we implemented a proteomic analyzing pipeline to identify proteins with multiple RRMs and predict their domain boundaries using specific PSSMs, domain architectures, and proteins with the same entity name. After clustering sequences on the basis of their evolutionary distances, a reference group is selected comparing domain architectures. Then, candidate proteins are collected in a proteome using specific PSSMs from seed alignments in PFAM. Finally, target proteins are identified using multiple alignments and phyolgenetic trees between candidate and reference proteins. Therefore, we identified 33 proteins close to 12 types of RRM containing proteins and their domain boundaries among 508 candidates from 33610 sequences in a human proteome. |
Year | DOI | Venue |
|---|---|---|
2011 | 10.1109/BIBMW.2011.6112401 | BIBM Workshops |
Keywords | Field | DocType |
multiple alignments,phyolgenetic trees,rna-recognition motif,rna-binding domain,human proteome,automated annotation tool,domain architecture,candidate protein,proteins,abundant rna-binding domain,post-transcriptional processes,similar domain architecture,domain boundaries,multiple rrms,biology computing,molecular configurations,domain architectures,clustering sequences,proteomic analyzing pipeline,proteomics,evolutionary distances,domain boundary,specific pssms,dna,proteomic analysis,multiple alignment,rna recognition motif | Architecture domain,Human proteome project,Annotation,Proteomics,Computer science,Proteome,Bioinformatics,Cluster analysis | Conference |
ISSN | ISBN | Citations |
2163-6966 | 978-1-4577-1612-6 | 0 |
PageRank | References | Authors |
0.34 | 7 | 5 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Kyung Dae Ko | 1 | 3 | 1.79 |
| Chun-Mei Liu | 2 | 245 | 41.30 |
| Mugizi Robert Rwebangira | 3 | 122 | 8.82 |
| Legand Burge | 4 | 29 | 9.60 |
| William Southerland | 5 | 15 | 6.23 |