Paper Info
Title
Whole Genome Sequencing, Comparative Genomics, and Systems Biology
Abstract
The whole-genome shotgun sequencing method with paired end-reads has proven rapid and economical, producing high-quality reconstructions of Drosophila (2000), human (2001) and mouse (2001), in quick succession. We discuss the overall algorithmic strategy, the results one can expect by comparing the whole genome assembly of Drosophila against the recently finished sequence, and advances such as high-density solid state sequencing and single molecule detection systems. We anticipate having the euchromatic portions of the genomes of twelve species of Drosophila in the next year. We discuss the current state of the art in comparative gene finding, cis-control module finding, and possible improvements. The hope of these approaches is that we will be able to accurately identify the "parts lists" of the D. melanogaster genome, a basic prerequisite for systems biology. We conclude with a segment on the possibility of a program of high-throughput in-situ image analysis in Drosophila embryos. We describe what information we might collect and what we might be able to infer from it. It is our contention that this may be the best way to understand development from a systems perspective.
Year
DOI
Venue
2004
10.1109/CSB.2004.168
CSB
Keywords
Field
DocType
high throughput,genetics,genome sequence,image analysis,embryos,comparative genomics,whole genome shotgun,gene finding,molecular biophysics,system biology
Genome,Shotgun sequencing,Hybrid genome assembly,Computer science,Systems biology,Gene prediction,Comparative genomics,Whole genome sequencing,Bioinformatics,Sequence assembly
Conference
ISBN
Citations 
PageRank 
0-7695-2194-0
0
0.34
References 
Authors
0
1
Name
Order
Citations
PageRank
Eugene Myers13164496.92