Name
Title | ||
|---|---|---|
Multiclass maximum-likelihood symmetry determination and motif reconstruction of 3-D helical objects from projection images for electron microscopy. |
Abstract | ||
|---|---|---|
Many micro- to nano-scale 3-D biological objects have a helical symmetry. Cryo electron microscopy provides 2-D projection images where, however, the images have low SNR and unknown projection directions. The object is described as a helical array of identical motifs, where both the parameters of the helical symmetry and the motif are unknown. Using a detailed image formation model, a maximum-likelihood estimator for the parameters of the symmetry and the 3-D motif based on images of many objects and algorithms for computing the estimate are described. The possibility that the objects are not identical but rather come from a small set of homogeneous classes is included. The first example is based on 316 128 × 100 pixel experimental images of Tobacco Mosaic Virus, has one class, and achieves 12.40-Å spatial resolution in the reconstruction. The second example is based on 400 128 × 128 pixel synthetic images of helical objects constructed from NaK ion channel pore macromolecular complexes, has two classes differing in helical symmetry, and achieves 7.84- and 7.90-Å spatial resolution in the reconstructions for the two classes. |
Year | DOI | Venue |
|---|---|---|
2011 | 10.1109/TIP.2011.2107329 | IEEE Transactions on Image Processing |
Keywords | Field | DocType |
cryoelectron microscopy,tobacco mosaic virus,cryo electron microscopy (cryo em),multiclass maximum-likelihood symmetry determination,biomembrane transport,maximum likelihood estimator,tomography,nanoscale 3d biological objects,pixel synthetic image,3-d biological object,multiclass maximum likelihood symmetry determination,tobacco mosaic virus (tmv),image resolution,maximum likelihood estimation,microorganisms,helical symmetry,helical object,motif reconstruction,spatial resolution,sodium,2-d projection image,ion channel pore macromolecular complexes,biological techniques,physiological models,biology computing,image reconstruction,virus,molecular biophysics,projection images,biothermics,microscale 3d biological objects,macromolecules,identical motif,na,potassium,3-d motif,3d helical objects,pixel experimental image,electron microscopy,3-d helical objects,maximum-likelihood (ml),k,helical array,fourier transforms,maximum likelihood,scattering,pixel,fourier transform,sodium channels,maximum likelihood estimate,microscopy,algorithms,potassium channels,ion channel,cryo electron microscopy | Iterative reconstruction,Pattern recognition,Helical antenna,Image processing,Image formation,Fourier transform,Artificial intelligence,Pixel,Cryo-electron microscopy,Image resolution,Mathematics | Journal |
Volume | Issue | ISSN |
20 | 7 | 1941-0042 |
Citations | PageRank | References |
0 | 0.34 | 8 |
Authors | ||
3 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Seunghee Lee | 1 | 42 | 8.53 |
| P. C. Doerschuk | 2 | 67 | 19.09 |
| J.E. Johnson | 3 | 27 | 5.49 |