Name
Abstract | ||
|---|---|---|
Array-based oligonucleotide synthesis technologies provide access to thousands of custom-designed sequence variants at low cost. Large-scale synthesis and high-throughput assays have become valuable experimental tools to study in detail the interplay between sequence and function. We have developed a methodology and corresponding algorithms for the design of diverse protein coding gene libraries, to exploit the potential of multiplex synthesis and help elucidate the effects of codon utilization and other factors in gene expression. Using our algorithm, we have computationally designed gene libraries with hundreds to thousands of orthogonal codon usage variants, uniformly exploring the design space of codon utilization, while demanding only a small fraction of the synthesis cost that would be required if these variants were synthesized independently. |
Year | DOI | Venue |
|---|---|---|
2012 | 10.1021/sb300086d | BCB |
Keywords | Field | DocType |
large-scale synthesis,orthogonal codon usage variant,rational design,codon utilization,design space,custom-designed sequence variant,multiplex synthesis,gene expression,synthesis cost,array-based oligonucleotide synthesis technology,orthogonal library,gene library,genomic libraries,synthetic biology | Gene,Biology,Multiplex,Oligonucleotide synthesis,Genomic library,Coding (social sciences),Computational biology,Genetics,Rational design,Synthetic biology,Codon usage bias | Conference |
Volume | Issue | ISSN |
2 | 5 | 2161-5063 |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
2 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Daniel Ryan | 1 | 0 | 0.34 |
| Dimitris Papamichail | 2 | 4 | 4.86 |