Paper Info
Title
Modular Sequence Elements Associated With Origin Regions In Eukaryotic Chromosomal Dna
Abstract
We have postulated that chromosomal replication origin regions in eukaryotes have in common clusters of certain modular sequence elements (Benbow, Zhao, and Larson, BioEssays 14, 661-670, 1992). In this study, computer analyses of DNA sequences from six origin regions showed that each contained one or more potential initiation regions consisting of a putative DUE (DNA unwinding element) aligned with clusters of SAR (scaffold associated region), and ARS (autonomously replicating sequence) consensus sequences, and pyrimidine tracts. The replication origins analyzed were from the following loci: Tetrahymena thermophila macronuclear rDNA gene, Chinese hamster ovary dihydrofolate reductase amplicon, human c-myc protooncogene, chicken histone H5 gene, Drosophila melanogaster chorion gene cluster on the third chromosome, and Chinese hamster ovary rhodopsin gene. The locations of putative initiation regions identified by the computer analyses were compared with published data obtained using diverse methods to map initiation sites. For at least four loci, the potential initiation regions identified by sequence analysis aligned with previously mapped initiation events. A consensus DNA sequence, WAWTTDDWW-WDHWGWHMAWTT, was found within the potential initiation regions in every case. An additional 35 kb of combined flanking sequences from the six loci were also analyzed, but no additional copies of this consensus sequence were found.
Year
DOI
Venue
1994
10.1093/nar/22.13.2479
NUCLEIC ACIDS RESEARCH
Field
DocType
Volume
Autonomously replicating sequence,Gene,Biology,Nucleic acid sequence,Scaffold-Associated Region,DNA unwinding element,Genetics,Locus (genetics),Molecular biology,Consensus sequence,Sequence analysis
Journal
22
Issue
ISSN
Citations 
13
0305-1048
0
PageRank 
References 
Authors
0.34
0
3
Name
Order
Citations
PageRank
Drena Dobbs142335.43
Wen-ling Shaiu215210.37
Robert M. Benbow300.34