Name
Title | ||
|---|---|---|
Colt-Cancer: Functional Genetic Screening Resource For Essential Genes In Human Cancer Cell Lines |
Abstract | ||
|---|---|---|
Genome-wide pooled shRNA screens enable global identification of the genes essential for cancer cell survival and proliferation and provide a 'functional genetic' map of human cancer to complement genomic studies. Using a lentiviral shRNA library targeting approximately 16 000 human genes and a newly developed scoring approach, we identified essential gene profiles in more than 70 breast, pancreatic and ovarian cancer cell lines. We developed a web-accessible database system for capturing information from each step in our standardized screening pipeline and a gene-centric search tool for exploring shRNA activities within a given cell line or across multiple cell lines. The database consists of a laboratory information and management system for tracking each step of a pooled shRNA screen as well as a web interface for querying and visualization of shRNA and gene-level performance across multiple cancer cell lines. COLT-Cancer Version 1.0 is currently accessible at http://colt.ccbr.utoronto.ca/cancer. |
Year | DOI | Venue |
|---|---|---|
2012 | 10.1093/nar/gkr959 | NUCLEIC ACIDS RESEARCH |
Keywords | Field | DocType |
rna interference | Genome,Biology,Cancer cell,Ovarian cancer,Human genome,Essential gene,Genetics,Molecular biology,Small hairpin RNA,RNA interference,Cancer | Journal |
Volume | Issue | ISSN |
40 | D1 | 0305-1048 |
Citations | PageRank | References |
6 | 0.68 | 7 |
Authors | ||
6 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| J L Koh | 1 | 110 | 14.39 |
| Kevin R. Brown | 2 | 170 | 11.98 |
| Azin Sayad | 3 | 6 | 0.68 |
| Dahlia Kasimer | 4 | 6 | 0.68 |
| Troy Ketela | 5 | 6 | 0.68 |
| Jason Moffat | 6 | 10 | 1.79 |