Name
Abstract | ||
|---|---|---|
A large proportion of proteins expressed in Escherichia coli form inclusion bodies and thus require renaturation to attain a functional conformation for analysis. In this process, identifying and optimizing the refolding conditions and methodology is often rate limiting. In order to address this problem, we have developed REFOLD, a web-accessible relational database containing the published methods employed in the refolding of recombinant proteins. Currently, REFOLD contains > 300 entries, which are heavily annotated such that the database can be searched via multiple parameters. We anticipate that REFOLD will continue to grow and eventually become a powerful tool for the optimization of protein renaturation. REFOLD is freely available at http://refold.med.monash.edu.au. |
Year | DOI | Venue |
|---|---|---|
2006 | 10.1093/nar/gkj080 | NUCLEIC ACIDS RESEARCH |
Keywords | Field | DocType |
internet,protein expression | Protein folding,Biology,Relational database,Protein expression,Molecular biology,Database,Limiting,Protein Renaturation | Journal |
Volume | Issue | ISSN |
34 | Database issue | 0305-1048 |
Citations | PageRank | References |
1 | 0.39 | 2 |
Authors | ||
11 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Michelle K M Chow | 1 | 1 | 0.39 |
| Abdullah A Amin | 2 | 4 | 0.98 |
| Kate F. Fulton | 3 | 13 | 1.87 |
| Thushan Fernando | 4 | 1 | 0.39 |
| Lawrence Kamau | 5 | 1 | 0.39 |
| Chris Batty | 6 | 1 | 0.39 |
| Michael Louca | 7 | 1 | 0.39 |
| Storm Ho | 8 | 1 | 0.39 |
| James C Whisstock | 9 | 93 | 7.90 |
| Stephen P. Bottomley | 10 | 12 | 1.70 |
| Ashley M Buckle | 11 | 32 | 7.06 |