Title
Tracking multiple particles in fluorescence microscopy images via probabilistic data association
Abstract
Tracking subcellular structures displayed as `particles' in fluorescence microscopy images yields quantitative descriptions of the underlying dynamical processes. We have developed an approach for tracking multiple fluorescent particles. Our approach includes a localization scheme using probabilistic data association that combines a top-down strategy driven by the Kalman filter and a bottom-up strategy using standard localization algorithms for fluorescent particles. The combined scheme yields multiple positions that are incorporated to the filter via a combined innovation. To track objects in close proximity, we introduce a support map that adjusts the association probabilities. By using the combined localization scheme in conjunction with the Kalman filter we integrate localization and position estimation. The approach has been successfully applied to synthetic images as well as to real microscopy image sequences and the performance has been quantified.
Year
DOI
Venue
2011
10.1109/ISBI.2011.5872786
Chicago, IL
Keywords
Field
DocType
Kalman filters,biological techniques,biology computing,cellular biophysics,fluorescence,image sequences,object tracking,optical microscopy,position measurement,probability,sensor fusion,Kalman filter,fluorescence microscopy imaging,image sequences,particle tracking,position estimation,probabilistic data association,standard localization algorithms,Biomedical imaging,microscopy images,tracking,virus particles
Computer vision,Fluorescence microscope,Pattern recognition,Medical imaging,Computer science,Kalman filter,Sensor fusion,Video tracking,Data association,Artificial intelligence,Microscopy,Probabilistic logic
Conference
ISSN
ISBN
Citations 
1945-7928 E-ISBN : 978-1-4244-4128-0
978-1-4244-4128-0
2
PageRank 
References 
Authors
0.37
16
5
Name
Order
Citations
PageRank
William J. Godinez19110.98
Marko Lampe2464.16
Roland Eils364470.09
Barbara Müller4173.02
Karl Rohr534048.69