Name
Title | ||
|---|---|---|
Adsorption Of Antibody Protein Onto Plasma-Polymerized Film Characterized By Atomic Force Microscopy And Quartz Crystal Microbalance |
Abstract | ||
|---|---|---|
Adsorption of antibody protein (anti-human IgG) onto plasma-polymerized thin films (PPF) with nanoscale thickness was characterized by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). The PPF surface is very flat (less than 1 nm roughness) and its properties (charge and wettability) can be easily changed while retaining the backbone structure. We focus on two types of surfaces: one is the pristine surface of hexamethyldisiloxane (HMDS) PPF (hydrophobic) and the other is an HMDS PPF surface with nitrogen-plasma treatment (hydrophilic and positive-charged surface). The AFM image showed that the antibody molecules were densely adsorbed onto both surfaces and individual antibody molecules could be observed. The QCM profiles show a corresponding tendency with the AFM images. These results indicate that the plasma polymerized film can be the suitable biointerface for the application of biosensor and bioassay. |
Year | DOI | Venue |
|---|---|---|
2007 | 10.1093/ietele/e90-c.3.649 | IEICE TRANSACTIONS ON ELECTRONICS |
Keywords | Field | DocType |
antibody, plasma-polymerized film, adsorption | Nanotechnology,Hexamethyldisiloxane,Quartz crystal microbalance,Wetting,Optics,Biointerface,Chemical engineering,Surface finish,Biosensor,Adsorption,Engineering,Thin film | Journal |
Volume | Issue | ISSN |
E90C | 3 | 0916-8524 |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
3 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| hitoshi | 1 | 1 | 4.01 |
| Satoshi Miura | 2 | 0 | 1.01 |
| Naoya Murata | 3 | 1 | 1.64 |