Name
Abstract | ||
|---|---|---|
We describe a convenient assay for rapid qualitative evaluation of hybridization/ligation fidelity. The approach uses randomized probe strands of DNA and restriction enzyme digestion after amplification of reaction products by the polymerase chain reaction (PCR), we report ligation efficiencies and fidelities of two DNA ligases, T;C DNA ligase and Thermus aquaticus (Taq) DNA ligase, over a range of temperatures. |
Year | DOI | Venue |
|---|---|---|
2000 | 10.1089/10665270050514963 | JOURNAL OF COMPUTATIONAL BIOLOGY |
Keywords | DocType | Volume |
DNA computation,ligation,DNA ligase,restriction enzymes,PCR | Journal | 7.0 |
Issue | ISSN | Citations |
6 | 1066-5277 | 1 |
PageRank | References | Authors |
0.35 | 3 | 3 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| dirk faulhammer | 1 | 108 | 15.16 |
| Richard J. Lipton | 2 | 6412 | 1796.57 |
| Laura F Landweber | 3 | 206 | 28.80 |