Name
Abstract | ||
|---|---|---|
Interleukin 2 (IL2) mRNA has a short half-life in the cytoplasm of T lymphocytes, relative to most mRNA. We have discovered a candidate ribonuclease to account for the rapid turnover of IL2 mRNA in the cytosol of the human T lymphocyte cell line Jurkat. In partially purified form, this RNase is about 7 times as active on IL2 as on beta-globin mRNA. Pancreatic RNase, by contrast, does not show a significant preference for IL2 mRNA. Neither 5' capping, nor polyadenylation of the substrate mRNAs affects their degradation by the IL2-selective mRNase, whose activity is optimal in 0.5 mM Mg++ and 100 mM potassium acetate. The mRNase behaves like a protein of molecular weight 60 - 70,000 on gel chromatography, and is unusual in that it is insensitive to placental RNase inhibitor (RNasin). The mRNase cleaves IL2 mRNA at a small number of sites in the coding region, and IL2 mRNA containing only the coding region and 36 nucleotides of the 3'-noncoding region competes efficiently with full-length IL2 mRNA for the mRNase, whereas beta-globin mRNA does not. |
Year | DOI | Venue |
|---|---|---|
1993 | 10.1093/nar/21.1.155 | NUCLEIC ACIDS RESEARCH |
Keywords | Field | DocType |
dna,cell line,kinetics | Jurkat cells,Biology,Pancreatic ribonuclease,Messenger RNP,Polyadenylation,Coding region,Messenger RNA,Genetics,RNase P,Molecular biology,Ribonuclease | Journal |
Volume | Issue | ISSN |
21 | 1 | 0305-1048 |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
3 | ||