Name
Title | ||
|---|---|---|
High-resolution mapping of neuronal activity using the lipophilic thallium chelate complex TlDDC: Protocol and validation of the method. |
Abstract | ||
|---|---|---|
In neurons the rate of K+-uptake increases with increasing activity. K+-analogues like the heavy metal ion thallium (Tl+) can be used, therefore, as tracers for imaging neuronal activity. However, when water-soluble Tl+-salts are injected systemically only minute amounts of the tracer enter the brain and the Tl+-uptake patterns are influenced by regional differences in blood–brain barrier (BBB) K+-permeability. We here show that the BBB-related limitations in using Tl+ for imaging neuronal activity are no longer present when the lipophilic Tl+ chelate complex thallium diethyldithiocarbamate (TlDDC) is applied. We systemically injected rodents with TlDDC and mapped the Tl+-distribution in the brain using an autometallographic (AMG) technique, a histochemical method for detecting heavy metals. We find that Tl+-doses for optimum AMG staining could be substantially reduced, and regional differences attributable to differences in BBB K+-permeability were no longer detectable, indicating that TlDDC crosses the BBB. At the cellular level, however, the Tl+-distribution was essentially the same as after injection of water-soluble Tl+-salts, indicating Tl+-release from TlDDC prior to neuronal or glial uptake. Upon sensory stimulation or intracortical microstimulation neuronal Tl+-uptake increased after TlDDC injection, upon muscimol treatment neuronal Tl+-uptake decreased. We present a protocol for mapping neuronal activity with cellular resolution, which is based on intravenous TlDDC injections during ongoing activity in unrestrained behaving animals and short stimulation times of 5 min. |
Year | DOI | Venue |
|---|---|---|
2010 | 10.1016/j.neuroimage.2009.08.012 | NeuroImage |
Keywords | Field | DocType |
Thallium,Diethyldithiocarbamate,Potassium,Blood–brain barrier,Imaging | Nuclear medicine,Premovement neuronal activity,Biophysics,Microstimulation,Cognitive psychology,Psychology,Muscimol,Chelation,Thallium,Sensory stimulation therapy,Stimulation,Blood–brain barrier | Journal |
Volume | Issue | ISSN |
49 | 1 | 1053-8119 |
Citations | PageRank | References |
1 | 0.45 | 2 |
Authors | ||
10 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Jürgen Goldschmidt | 1 | 5 | 1.72 |
| Tim Wanger | 2 | 1 | 0.45 |
| Achim Engelhorn | 3 | 1 | 0.45 |
| Hergen Friedrich | 4 | 1 | 0.45 |
| Max Happel | 5 | 1 | 0.45 |
| Anton Ilango | 6 | 1 | 0.45 |
| Mario Engelmann | 7 | 1 | 0.45 |
| Ingo W Stuermer | 8 | 1 | 0.45 |
| Frank W. Ohl | 9 | 31 | 5.04 |
| Henning Scheich | 10 | 147 | 29.04 |