Title
Resolving the three-dimensional histology of the heart
Abstract
Cardiac histo-anatomical structure is a key determinant in all aspects of cardiac function. While some characteristics of micro- and macrostructure can be quantified using non-invasive imaging methods, histology is still the modality that provides the best combination of resolution and identification of cellular/sub-cellular substrate identities. The main limitation of histology is that it does not provide inherently consistent three-dimensional (3D) volume representations. This paper presents methods developed within our group to reconstruct 3D histological datasets. It includes the use of high-resolution MRI and block-face images to provide supporting volumetric datasets to guide spatial reintegration of 2D histological section data, and presents recent developments in sample preparation, data acquisition, and image processing.
Year
DOI
Venue
2012
10.1007/978-3-642-33636-2_2
CMSB
Keywords
DocType
Citations 
three-dimensional histology,histological section data,data acquisition,image processing,cardiac histo-anatomical structure,histological datasets,cardiac function,high-resolution mri,block-face image,best combination,volumetric datasets
Conference
0
PageRank 
References 
Authors
0.34
2
11