Title
PhylOPDb: a 16S rRNA oligonucleotide probe database for prokaryotic identification.
Abstract
In recent years, high-throughput molecular tools have led to an exponential growth of available 16S rRNA gene sequences. Incorporating such data, molecular tools based on target-probe hybridization were developed to monitor microbial communities within complex environments. Unfortunately, only a few 16S rRNA gene-targeted probe collections were described. Here, we present PhylOPDb, an online resource for a comprehensive phylogenetic oligonucleotide probe database. PhylOPDb provides a convivial and easy-to-use web interface to browse both regular and explorative 16S rRNA-targeted probes. Such probes set or subset could be used to globally monitor known and unknown prokaryotic communities through various techniques including DNA microarrays, polymerase chain reaction (PCR), fluorescent in situ hybridization (FISH), targeted gene capture or in silico rapid sequence identification. PhylOPDb contains 74 003 25-mer probes targeting 2178 genera including Bacteria and Archaea. Database URL: http://g2im.u-clermont1.fr/phylopdb/
Year
DOI
Venue
2014
10.1093/database/bau036
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
Keywords
Field
DocType
bacteria,computer simulation
Phylogenetic tree,Archaea,Polymerase chain reaction,Gene,Biology,16S ribosomal RNA,Bioinformatics,Oligomer restriction,DNA microarray,Database,In silico
Journal
Volume
Issue
ISSN
2014
0
1758-0463
Citations 
PageRank 
References 
1
0.37
9
Authors
12