Paper Info
Title
Experimental Validation of Signal Dependent Operation in Whiplash PCR
Abstract
Whiplash PCR (WPCR), which implements self-directed operation, programmed within a single DNA molecule, is a potential candidate for both mathematical and biological applications. However, WPCR-based methods are known to suffer from a serious efficiency problem called back-hybridization (BH). Previously, we proposed and partially validated a new rule-protect operation to abolish BH. In this work, we experimentally demonstrate the ability of rule-protect to drive multi-step WPCR. Successful implementation of isothermal operation at physiological temperatures is an essential benchmark for biological applications. We also propose the use of rule-protect for external signalling to control computational operation. Consequently, signal-dependent self-directed operation, which is conceptually new to DNA computing, is achieved. The present architecture, provided with sensing ability, allows a composite system design layering computational reactions, and would be suitable for functioning as the central processing unit of this system.
Year
DOI
Venue
2008
10.1007/978-3-642-03076-5_1
DNA Computing
Keywords
DocType
Volume
computational operation,experimental validation,signal-dependent self-directed operation,composite system design,computational reaction,isothermal operation,dna computing,multi-step wpcr,signal dependent operation,whiplash pcr,self-directed operation,biological application,new rule-protect operation
Conference
5347
ISSN
Citations 
PageRank 
0302-9743
2
0.41
References 
Authors
5
3
Name
Order
Citations
PageRank
Ken Komiya155.38
Masayuki Yamamura224237.62
John A. Rose314026.22