Paper Info
Title
Human Box C/D Snornas With Mirna Like Functions: Expanding The Range Of Regulatory Rnas
Abstract
Small nucleolar RNAs (snoRNAs) and microRNAs are two classes of non-protein-coding RNAs with distinct functions in RNA modification or post-transcriptional gene silencing. In this study, we introduce novel insights to RNA-induced gene activity adjustments in human cells by identifying numerous snoRNA-derived molecules with miRNA-like function, including H/ACA box snoRNAs and C/D box snoRNAs. In particular, we demonstrate that several C/D box snoRNAs give rise to gene regulatory RNAs, named sno-miRNAs here. Our data are complementing the increasing number of studies in the field of small RNAs with regulatory functions. In massively deep sequencing of small RNA fractions we identified high copy numbers of sub-sequences from > 30 snoRNAs with lengths of epsilon 18 nt. RNA secondary structure prediction indicated for a majority of candidates a location in predicted stem regions. Experimental analysis revealed efficient gene silencing for 11 box C/D sno-miRNAs, indicating cytoplasmic processing and recruitment to the RNA silencing machinery. Assays in four different human cell lines indicated variations in both the snoRNA levels and their processing to active sno-miRNAs. In addition we show that box D elements are predominantly flanking at least one of the sno-miRNA strands, while the box C element locates within the sequence of the sno-miRNA guide strand.
Year
DOI
Venue
2011
10.1093/nar/gkq776
NUCLEIC ACIDS RESEARCH
Field
DocType
Volume
Long non-coding RNA,Trans-acting siRNA,RNA,RNA silencing,Small RNA,Biology,Small nucleolar RNA,Argonaute,Genetics,Molecular biology,Non-coding RNA
Journal
39
Issue
ISSN
Citations 
2
0305-1048
2
PageRank 
References 
Authors
0.52
4
5
Name
Order
Citations
PageRank
Markus Brameier141528.94
Astrid Herwig220.52
Richard Reinhardt3242.82
Lutz Walter420.52
Jens Gruber520.52