Name
Abstract | ||
|---|---|---|
Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the 3' end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, designs the primers, lists the appropriate restriction enzymes and other related information. |
Year | DOI | Venue |
|---|---|---|
2001 | 10.1093/bioinformatics/17.9.838 | BIOINFORMATICS |
Keywords | Field | DocType |
single nucleotide polymorphism | Amplified fragment length polymorphism,Polymerase chain reaction,Biology,Restriction fragment length polymorphism,Primer (molecular biology),Single-nucleotide polymorphism,Terminal restriction fragment length polymorphism,Bioinformatics,Genetics,Restriction enzyme,Restriction map | Journal |
Volume | Issue | ISSN |
17 | 9 | 1367-4803 |
Citations | PageRank | References |
4 | 0.89 | 1 |
Authors | ||
3 | ||