Title
PESTAS: a web server for EST analysis and sequence mining.
Abstract
We have developed a web server for the high-throughput annotation of expressed sequence tags (ESTs) called pipeline for EST analysis service (PESTAS). PESTAS processes entire datasets with an automated pipeline of 13 analytic services, then deposits the data into the MySQL database and transforms it into three kinds of reports: preprocessing, assembling and annotation. All annotated information is provided to the scientist and can be downloaded through a web browser. To get more relevant functional annotation results, a curation function was introduced with which biologists can easily change the best-hit annotation information. We included a gene chip module that detects gene expression differences between libraries by comparing accession number counts from BLAST search results. PESTAS also provides access to the pathway information of KEGG, which is useful for mapping the relationships among networks of annotated enzymes, and is especially valuable for those researchers interested in biological pathways.
Year
DOI
Venue
2009
10.1093/bioinformatics/btp293
BIOINFORMATICS
Keywords
Field
DocType
sequence mining
Data mining,World Wide Web,Annotation,Expressed sequence tag,Web browser,Computer science,KEGG,Preprocessor,Bioinformatics,Sequential Pattern Mining,Web server
Journal
Volume
Issue
ISSN
25
14
1367-4803
Citations 
PageRank 
References 
0
0.34
7
Authors
8
Name
Order
Citations
PageRank
Seong-Hyeuk Nam100.68
Dae-Won Kim200.34
Tae-Sung Jung35818.80
Youngsang Choi471.86
Dong Wook Kim57622.66
Han-Suk Choi601.69
Sang-Haeng Choi700.68
Hong-seog Park801.35