Paper Info
Title
Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo.
Abstract
We have demonstrated the feasibility of imaging mouse brain metabolism using photoacoustic computed tomography (PACT), a fast, noninvasive and functional imaging modality with optical contrast and acoustic resolution. Brain responses to forepaw stimulations were imaged transdermally and transcranially. 2-NBDG, which diffuses well across the blood–brain-barrier, provided exogenous contrast for photoacoustic imaging of glucose response. Concurrently, hemoglobin provided endogenous contrast for photoacoustic imaging of hemodynamic response. Glucose and hemodynamic responses were quantitatively decoupled by using two-wavelength measurements. We found that glucose uptake and blood perfusion around the somatosensory region of the contralateral hemisphere were both increased by stimulations, indicating elevated neuron activity. While the glucose response area was more homogenous and confined within the somatosensory region, the hemodynamic response area had a clear vascular pattern and spread wider than the somatosensory region. Our results demonstrate that 2-NBDG-enhanced PACT is a promising tool for noninvasive studies of brain metabolism.
Year
DOI
Venue
2013
10.1016/j.neuroimage.2012.08.054
NeuroImage
Keywords
Field
DocType
Photoacoustic computed tomography,Brain imaging,2-NBDG,Forepaw stimulation,Glucose metabolism,Hemodynamic response
Biomedical engineering,Hemodynamics,Glucose uptake,Fluorescence-lifetime imaging microscopy,Haemodynamic response,Functional imaging,Optics,Medical physics,Somatosensory system,Neuroimaging,Perfusion,Physics
Journal
Volume
ISSN
Citations 
64
1053-8119
16
PageRank 
References 
Authors
1.40
1
7
Name
Order
Citations
PageRank
Junjie Yao1183.10
Jun Xia2161.40
Konstantin Maslov3182.30
Mohammadreza Nasiriavanaki4275.27
Vassiliy Tsytsarev5323.41
Alexei V Demchenko6161.40
Lihong V Wang719458.39