Paper Info
Title
Quality control in manufacturing oligo arrays: a combinatorial design approach.
Abstract
The advent of the DNA microarray technology has brought with it the exciting possibility of simultaneously observing the expression levels of all genes in an organism. One such microarray technology, called "oligo arrays," manufactures short single strands of DNA (called probes) onto a glass surface using photolithography. An altered or missed step in such a manufacturing protocol can adversely affect all probes using this failed step and is in general impossible to disentangle from experimental variation when using such a defective array. The idea of designing special quality control probes to detect a failed step was first formulated by Hubbell and Pevzner (1999). We consider an alternative formulation of this problem and use a combinatorial design approach to solve it. Our results improve over prior work in guaranteeing coverage of all protocol steps and in being able to tolerate a greater number of unreliable probe intensities.
Year
DOI
Venue
2001
10.1089/10665270252833163
JOURNAL OF COMPUTATIONAL BIOLOGY
Keywords
DocType
Volume
DNA microarray,oligo array,photolithography,quality control,combinatorial design,error-correcting code
Conference
9.0
Issue
ISSN
Citations 
1
1066-5277
11
PageRank 
References 
Authors
1.33
3
2
Name
Order
Citations
PageRank
Rimli Sengupta113938.86
Martin Tompa2111.33