Paper Info
Title
Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device
Abstract
Microfiltration is an important microfluidic technique suitable for enrichment and isolation of cells. However, cell lysing could occur due to hydrodynamic damage that may be detrimental for medical diagnostics. Therefore, we conducted a systematic study of hydrodynamic cell lysing in a high-throughput Circular Multi-Channel Microfiltration (CMCM) device integrated with a polycarbonate membrane. HeLa cells (cervical cancer cells) were driven into the CMCM at different flow rates. The viability of the cells in the CMCM was examined by fluorescence microscopy using Acridine Orange (AO)/Ethidium Bromide (EB) as a marker for viable/dead cells. A simple analytical cell viability model was derived and a 3D numerical model was constructed to examine the correlation of between cell lysing and applied shear stress under varying flow rate and Reynolds number. The measured cell viability as a function of the shear stress was consistent with theoretical and numerical predictions when accounting for cell size distribution. © 2013 IEEE.
Year
DOI
Venue
2013
10.1109/NEMS.2013.6559761
Nano/Micro Engineered and Molecular Systems
Keywords
DocType
Volume
cell lysing,cell viability,enrichment,hydrodynamic shear,isolation,microfiltration,microfluidics,cancer,fluorescence microscopy,fluorescence,stress,medical diagnostics,reynolds number,gynaecology,hydrodynamics,numerical analysis,shear stress
Conference
null
Issue
ISSN
ISBN
null
null
978-1-4673-6351-8
Citations 
PageRank 
References 
1
0.85
0
Authors
7
Name
Order
Citations
PageRank
W. Ma110.85
D. Liu218012.77
Hooman Shagoshtasbi310.85
Amit Shukla410.85
E. S. Nugroho510.85
Yitshak Zohar610.85
Yi-Kuen Lee7711.16