Title | ||
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Simultaneous Vision-Based Shape and Motion Analysis of Cells Fast-Flowing in a Microchannel |
Abstract | ||
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This paper proposes a novel concept for simultaneous cell shape and motion analysis in fast microchannel flows by implementing a multiobject feature extraction algorithm on a frame-straddling high-speed vision platform. The system can synchronize two camera inputs with the same view with only a tiny time delay on the sub-microsecond timescale. Real-time video processing is performed in hardware logic by extracting the moment features of multiple cells in 512 × 256 images at 4000 fps for the two camera inputs and their frame-straddling time can be adjusted from 0 to 0.25 ms in 9.9 ns steps. By setting the frame-straddling time in a certain range to avoid large image displacements between the two camera inputs, our frame-straddling high-speed vision platform can perform simultaneous shape and motion analysis of cells in fast microchannel flows of 1 m/s or greater. The results of real-time experiments conducted to analyze the deformabilities and velocities of sea urchin egg cells fast-flowing in microchannels verify the efficacy of our vision-based cell analysis system. |
Year | DOI | Venue |
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2015 | 10.1109/TASE.2013.2292583 | IEEE T. Automation Science and Engineering |
Keywords | Field | DocType |
Microchannel,Cameras,Shape,Real-time systems,Feature extraction,Head,Delay effects | Computer vision,Synchronization,Video processing,Feature extraction algorithm,Computer science,Vision based,Feature extraction,Microchannel,Artificial intelligence,Motion analysis | Journal |
Volume | Issue | ISSN |
12 | 1 | 1545-5955 |
Citations | PageRank | References |
10 | 0.75 | 9 |
Authors | ||
4 |
Name | Order | Citations | PageRank |
---|---|---|---|
Qingyi Gu | 1 | 153 | 22.13 |
Tadayoshi Aoyama | 2 | 69 | 28.58 |
Takeshi Takaki | 3 | 222 | 38.04 |
Idaku Ishii | 4 | 355 | 64.37 |