Title
An image processing approach to analyze morphological features of microscopic images of muscle fibers.
Abstract
We present an image processing approach to automatically analyze duo-channel microscopic images of muscular fiber nuclei and cytoplasm. Nuclei and cytoplasm play a critical role in determining the health and functioning of muscular fibers as changes of nuclei and cytoplasm manifest in many diseases such as muscular dystrophy and hypertrophy. Quantitative evaluation of muscle fiber nuclei and cytoplasm thus is of great importance to researchers in musculoskeletal studies. The proposed computational approach consists of steps of image processing to segment and delineate cytoplasm and identify nuclei in two-channel images. Morphological operations like skeletonization is applied to extract the length of cytoplasm for quantification. We tested the approach on real images and found that it can achieve high accuracy, objectivity, and robustness.
Year
DOI
Venue
2014
10.1016/j.compmedimag.2014.07.003
Computerized Medical Imaging and Graphics
Keywords
DocType
Volume
Cytoplasm,Nuclei,Skeletonization,Quantification,Segmentation,Muscle fiber
Journal
38
Issue
ISSN
Citations 
8
0895-6111
1
PageRank 
References 
Authors
0.37
1
5
Name
Order
Citations
PageRank
Cesar Henrique Comin110.70
Xiaoyin Xu219222.19
Yaming Wang310.37
Luciano da F. Costa435734.63
Zhong Yang5202.29