Paper Info
Title
PTEN hopping on the cell membrane is regulated via a positively-charged C2 domain.
Abstract
PTEN, a tumor suppressor that is frequently mutated in a wide spectrum of cancers, exerts PI(3,4,5) P-3 phosphatase activities that are regulated by its dynamic shuttling between the membrane and cytoplasm. Direct observation of PTEN in the interfacial environment can offer quantitative information about the shuttling dynamics, but remains elusive. Here we show that positively charged residues located in the c alpha 2 helix of the C2 domain are necessary for the membrane localization of PTEN via stable electrostatic interactions in Dictyostelium discoideum. Single-molecule imaging analyses revealed that PTEN molecules moved distances much larger than expected had they been caused by lateral diffusion, a phenomenon we call "hopping." Our novel single-particle tracking analysis method found that the c alpha 2 helix aids in regulating the hopping and stable-binding states. The dynamically established membrane localization of PTEN was revealed to be essential for developmental processes and clarified a fundamental regulation mechanism of the protein quantity and activity on the plasma membrane.
Year
DOI
Venue
2014
10.1371/journal.pcbi.1003817
PLOS COMPUTATIONAL BIOLOGY
DocType
Volume
Issue
Journal
10
9
ISSN
Citations 
PageRank 
1553-7358
0
0.34
References 
Authors
1
3
Name
Order
Citations
PageRank
Masato Yasui131.42
Satomi Matsuoka242.85
Masahiro Ueda3276.49