Name
Title | ||
|---|---|---|
Restricted N-glycan conformational space in the PDB and its implication in glycan structure modeling. |
Abstract | ||
|---|---|---|
Understanding glycan structure and dynamics is central to understanding protein-carbohydrate recognition and its role in protein-protein interactions. Given the difficulties in obtaining the glycan's crystal structure in glycoconjugates due to its flexibility and heterogeneity, computational modeling could play an important role in providing glycosylated protein structure models. To address if glycan structures available in the PDB can be used as templates or fragments for glycan modeling, we present a survey of the N-glycan structures of 35 different sequences in the PDB. Our statistical analysis shows that the N-glycan structures found on homologous glycoproteins are significantly conserved compared to the random background, suggesting that N-glycan chains can be confidently modeled with template glycan structures whose parent glycoproteins share sequence similarity. On the other hand, N-glycan structures found on non-homologous glycoproteins do not show significant global structural similarity. Nonetheless, the internal substructures of these N-glycans, particularly, the substructures that are closer to the protein, show significantly similar structures, suggesting that such substructures can be used as fragments in glycan modeling. Increased interactions with protein might be responsible for the restricted conformational space of N-glycan chains. Our results suggest that structure prediction/modeling of N-glycans of glycoconjugates using structure database could be effective and different modeling approaches would be needed depending on the availability of template structures. |
Year | DOI | Venue |
|---|---|---|
2013 | 10.1371/journal.pcbi.1002946 | PLOS COMPUTATIONAL BIOLOGY |
Keywords | Field | DocType |
protein structure,glycoproteins,crystal structure,protein structure prediction | Protein structure prediction,Biology,Glycoconjugate,Glycoprotein,Structural similarity,Bioinformatics,Template,Protein Data Bank (RCSB PDB),Glycan,Protein structure | Journal |
Volume | Issue | ISSN |
9 | 3 | 1553-734X |
Citations | PageRank | References |
2 | 0.38 | 11 |
Authors | ||
4 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Sunhwan Jo | 1 | 134 | 13.08 |
| Hui Sun Lee | 2 | 41 | 5.66 |
| Jeffrey Skolnick | 3 | 135 | 24.44 |
| Wonpil Im | 4 | 132 | 21.26 |