Title
NucleusJ: an ImageJ plugin for quantifying 3D images of interphase nuclei.
Abstract
A Summary: NucleusJ is a simple and user-friendly ImageJ plugin dedicated to the characterization of nuclear morphology and chromatin organization in 3D. Starting from image stacks, the nuclear boundary is delimited by combining the Otsu segmentation method with optimization of nuclear sphericity. Chromatin domains are segmented by partitioning the nucleus using a 3D watershed algorithm and by thresholding a contrast measure over the resulting regions. As output, NucleusJ quantifies 15 parameters including shape and size of nuclei as well as intra-nuclear objects and their position within the nucleus. A step-by-step documentation is available for self-training, together with data sets of nuclei with different nuclear organization.
Year
DOI
Venue
2015
10.1093/bioinformatics/btu774
BIOINFORMATICS
Field
DocType
Volume
Nucleus,Data set,Computer science,Segmentation,Thresholding,Bioinformatics,Plug-in,Interphase,Abstract Summary
Journal
31
Issue
ISSN
Citations 
7
1367-4803
1
PageRank 
References 
Authors
0.37
4
6
Name
Order
Citations
PageRank
Axel Poulet110.37
Ignacio Arganda-Carreras2718.02
David Legland310.70
Aline V Probst410.37
Philippe Andrey515320.54
Christophe Tatout610.37