Name
Abstract | ||
|---|---|---|
Colicin E7 is a natural bacterial toxin. Its nuclease domain (NColE7) enters the target cell and kills it by digesting the nucleic acids. The HNH-motif as the catalytic centre of NColE7 at the C-terminus requires the positively charged N-terminal loop for the nuclease activity-offering opportunities for allosteric control in a NColE7-based artificial nuclease. Accordingly, four novel zinc finger nucleases were designed by computational methods exploiting the special structural features of NColE7. The constructed models were subjected to MD simulations. The comparison of structural stability and functional aspects showed that these models may function as safely controlled artificial nucleases. This study was complemented by random mutagenesis experiments identifying potentially important residues for NColE7 function outside the catalytic region. |
Year | DOI | Venue |
|---|---|---|
2014 | 10.1007/s10822-014-9765-8 | Journal of computer-aided molecular design |
Keywords | Field | DocType |
Metalloenzyme,Zinc finger nuclease,Colicin E7,Computational protein design | Biochemistry,Colicin,Nuclease,Chemistry,Allosteric regulation,Zinc finger nuclease,Nucleic acid,Bioinformatics,Mutagenesis,Zinc finger,Peptide sequence | Journal |
Volume | Issue | ISSN |
28 | 8 | 1573-4951 |
Citations | PageRank | References |
0 | 0.34 | 3 |
Authors | ||
6 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Eszter Németh | 1 | 0 | 0.34 |
| Gabriella K Schilli | 2 | 0 | 0.34 |
| Gábor Nagy | 3 | 0 | 0.34 |
| Christoph Hasenhindl | 4 | 0 | 0.34 |
| Béla Gyurcsik | 5 | 0 | 0.34 |
| Chris Oostenbrink | 6 | 297 | 38.41 |