Paper Info
Title
An investigation into eukaryotic pseudouridine synthases.
Abstract
A common post-transcriptional modification of RNA is the conversion of uridine to its isomer pseudouridine. We investigated the biological significance of eukaryotic pseudouridine synthases using the yeast Saccharomyces cerevisiae. We conducted a comprehensive statistical analysis on growth data from automated perturbation (gene deletion) experiments, and used bi-logistic curve analysis to characterise the yeast phenotypes. The deletant strains displayed different alteration in growth properties, including in some cases enhanced growth and/or biphasic growth curves not seen in wild-type strains under matched conditions. These results demonstrate that disrupting pseudouridine synthases can have a significant qualitative erect on growth. We further investigated the significance of post-transcriptional pseudouridine modification through investigation of the scientific literature. We found that (1) In Toxoplasma gondii, a pseudouridine synthase gene is critical in cellular differentiation between the two asexual forms: Tachyzoites and bradyzoites; (2) Mutation of pseudouridine synthase genes has also been implicated in human diseases (mitochondrial myopathy and sideroblastic anemia (MLASA); dyskeratosis congenita). Taken together, these results are consistent with pseudouridine synthases having a Gene Ontology function of "biological regulation".
Year
DOI
Venue
2014
10.1142/S0219720014500152
JOURNAL OF BIOINFORMATICS AND COMPUTATIONAL BIOLOGY
Keywords
Field
DocType
RNA,Saccharomyces cerevisiae,growth analysis,biological regulation
Uridine,RNA,Gene,Biology,Biochemistry,Cellular differentiation,Yeast,Saccharomyces cerevisiae,Pseudouridine,Molecular biology,Biological regulation
Journal
Volume
Issue
ISSN
12
4
0219-7200
Citations 
PageRank 
References 
0
0.34
1
Authors
2
Name
Order
Citations
PageRank
Ross D. King11774194.85
Chuan Lu200.68