Name
Title | ||
|---|---|---|
Design and implementation of a biomolecular circuit for tracking protein concentration. |
Abstract | ||
|---|---|---|
This paper describes the design, modeling, synthesis and preliminary validation of a protein concentration regulator circuit. The circuit is designed to maintain the level of a target protein to a reference level, specified by the amount of another protein. This is implemented using a single negative feedback loop that inhibits the production of the target protein once its concentration is equal to the reference amount. A mathematical model consisting of a set of ODEs is derived from mass action laws and Hill function approximations of protein production. Steady-state analysis of the model is used to predict parameter sensitivity and experimental behavior. We implemented this circuit in E. coli using scaffold-based sequestration and transcriptional activation. Preliminary experimental results show the system matching predictions from our model and performing the expected task. |
Year | DOI | Venue |
|---|---|---|
2013 | 10.1109/ACC.2013.6580175 | ACC |
Keywords | Field | DocType |
ode,steady state analysis,molecular biophysics,differential equations,biochemistry,steady state,biocontrol,mathematical model,production,ordinary differential equation,proteins,negative feedback | Differential equation,Regulator,Function approximation,Computer science,Control theory,Target protein,Negative feedback,Control engineering,Molecular biophysics,Ode | Conference |
ISSN | Citations | PageRank |
0743-1619 | 1 | 0.38 |
References | Authors | |
1 | 3 | |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Emmanuel L. C. de los Santos | 1 | 1 | 0.38 |
| Victoria Hsiao | 2 | 1 | 0.72 |
| Richard M. Murray | 3 | 12322 | 1223.70 |