Name
Title | ||
|---|---|---|
A lab-on-chip approach for monitoring the electrochemical activity of biorealistic cell cultures |
Abstract | ||
|---|---|---|
The objective of any cell culturing platform is to decipher the in vivo functionality of native tissue in order to deliver reliable cell models for disease and pharmacological studies and eventually in patient-specific tissue engineering. We present a new perspective in lab-on-chip implementations for cell culturing, emphasizing on a versatile technology for cell micropatterning that can integrate electrical and pH monitoring modalities to record extracellular activity. We employ Parylene C, a highly biocompatible material, as a flexible culture substrate that controls the cellular microtopography and promotes a more in vivo-like morphology of neonatal rat ventricular myocytes. Moreover, we transfer the patterning technology on commercially available Multi-Electrode arrays to highlight the potential of integration with products customly used for extracellular electrical recordings. Finally, we implement flexible Parylene sensors for spatiotemporal pH monitoring, using the material both as a support medium and as a sensing membrane. Integration of these three attributes may deliver a compact solution with high scientific and commercial impact. |
Year | DOI | Venue |
|---|---|---|
2014 | 10.1109/ISCAS.2014.6865217 | ISCAS |
Keywords | Field | DocType |
cell micropatterning,electrical monitoring modalities,cellular biophysics,compact solution,diseases,cell culturing platform,ph monitoring modalities,electrical monitoring,biomedical electrodes,biomedical measurement,in vivo-like morphology,lab-on-chip implementations,pharmacological studies,tissue engineering,electrochemical electrodes,patient-specific tissue engineering,parylene c,extracellular electrical recordings,spatiotemporal ph monitoring,native tissue,sensing membrane,support medium,bioelectric phenomena,patterning technology,biocompatible material,multielectrode arrays,extracellular activity,neonatal rat ventricular myocytes,ph,lab-on-a-chip,lab-on-chip,cell models,electrochemical sensors,biorealistic cell cultures,biological tissues,flexible parylene sensor,disease studies,ph sensing,in vivo functionality,flexible culture substrate,cellular microtopography,electrochemical activity monitoring,lab on a chip,sensors,extracellular,electrodes,computer architecture,films,lab on chip | Biomedical engineering,Cell culture,Computer science,Electronic engineering,Tissue engineering,Extracellular,Biocompatible material,Micropatterning,Parylene,Lab-on-a-chip | Conference |
ISSN | Citations | PageRank |
0271-4302 | 0 | 0.34 |
References | Authors | |
0 | 6 | |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Tatiana Trantidou | 1 | 1 | 1.16 |
| Tatiana Tariq | 2 | 0 | 0.34 |
| K. Pinto | 3 | 0 | 0.34 |
| Christofer Toumazou | 4 | 265 | 59.06 |
| Cesare M. Terracciano | 5 | 0 | 0.34 |
| Themistoklis Prodromakis | 6 | 111 | 23.34 |