Title
Real-time LOC-based morphological cell analysis system using high-speed vision
Abstract
In this paper, a high-speed vision-based morphological analysis system for fast-flowing cells in a microchannel implementing a multi-object feature extraction algorithm on a high-speed vision platform is proposed. Real-time video processing is performed in hardware logic by extracting the moment features and bounding boxes of multiple cells in 512×256-pixel images at 2000 fps. The extracted cell regions are pushed into a first-in-first-out (FIFO) buffer for real-time image-based morphological analysis after being shrunk proportionally to a certain size. By extracting the bounding boxes of the cell regions using hardware logic and shrinking the cell region to a certain size to reduce processing time, our high-speed vision system can perform fast morphological analysis of cells at 2 ms/cell in fast microchannel flows. The results of real-time experiments conducted to analyze the size, eccentricity, and transparency of fertilized sea urchin eggs fast flowing in microchannels verify the efficacy of our vision-based cell analysis system.
Year
DOI
Venue
2014
10.1109/IROS.2014.6942654
IROS
Keywords
DocType
ISSN
video signal processing,cellular biophysics,fast-flowing cell,hardware logic,real-time LOC-based morphological cell analysis system,extracted cell region,bounding boxes,multiobject feature extraction algorithm,high-speed vision platform,biology computing,feature extraction,eccentricity,vision-based cell analysis system,computer vision,high-speed vision system,transparency,cell regions,FIFO buffer,microchannel flow,high-speed vision-based morphological analysis system,real-time video processing,real-time image-based morphological analysis,real-time systems,medical image processing,first-in-first-out buffer,fertilized sea urchin egg,microchannels
Conference
2153-0858
Citations 
PageRank 
References 
0
0.34
0
Authors
6
Name
Order
Citations
PageRank
Qingyi Gu115322.13
Tadayoshi Aoyama26928.58
Takeshi Takaki322238.04
Idaku Ishii435564.37
Ayumi Takemoto520.73
Naoaki Sakamoto620.73